Journal: Microbiology Resource Announcements

Article Title: Draft Genome Sequence of Rhodotorula mucilaginosa from an Adult Patient in Qatar

doi: 10.1128/MRA.00725-21

Figure Lengend Snippet: Rhodotorula mucilaginosa genome statistics and information

Article Snippet: Here, we present a draft genome assembly of R. mucilaginosa (Rhodo3571) from an immunocompetent host, possibly associated with central venous catheter (CVC) infection at Hamad Medical Corporation (Doha, Qatar) ( ).

Techniques:

Journal: Microbiology Resource Announcements

Article Title: Draft Genome Sequence of Rhodotorula mucilaginosa from an Adult Patient in Qatar

doi: 10.1128/MRA.00725-21

Figure Lengend Snippet: Genetic relationship among R. mucilaginosa available genomes based on core genome SNPs (rooted at the midpoint).

Article Snippet: Here, we present a draft genome assembly of R. mucilaginosa (Rhodo3571) from an immunocompetent host, possibly associated with central venous catheter (CVC) infection at Hamad Medical Corporation (Doha, Qatar) ( ).

Techniques:

Journal: The European Respiratory Journal

Article Title: R othia mucilaginosa is an anti-inflammatory bacterium in the respiratory tract of patients with chronic lung disease

doi: 10.1183/13993003.01293-2021

Figure Lengend Snippet: Overview of strains used in this study

Article Snippet: R. mucilaginosa absolute abundance was measured by quantitative PCR (qPCR) in 82 out of 85 samples using the Qiagen Microbial DNA qPCR Assay for R. mucilaginosa (BPID00297A; Qiagen, Hilden, Germany).

Techniques:

Journal: The European Respiratory Journal

Article Title: R othia mucilaginosa is an anti-inflammatory bacterium in the respiratory tract of patients with chronic lung disease

doi: 10.1183/13993003.01293-2021

Figure Lengend Snippet: Three-dimensional (3D) lung epithelial responses to pro-inflammatory stimuli in the presence and absence of members of the lung microbiota: Pseudomonas aeruginosa PAO1 (Pa), Rothia mucilaginosa DSM20746 (Rm), Staphylococcus aureus SP123 (S), Streptococcus anginosus LMG14696 (St), Achromobacter xylosoxidans LMG26680 (A) and Gemella haemolysans LMG18984 (G). a, b) Interleukin (IL)-8 production by 3D A549 cells after 4 h exposure to a) single bacterial cultures or b) co-cultures of various lung microbiota members with P. aeruginosa PAO1 at an MOI of 30:1. c) IL-8 production by 3D A549 cells after 4 or 24 h exposure to 100 μg·mL −1 lipopolysaccharide (LPS) alone or in co-culture with R. mucilaginosa at an MOI 10:1 (4 h) or 1:1 (24 h). d) IL-6, IL-8, granulocyte–macrophage colony-stimulating factor (GM-CSF) and monocyte chemoattractant protein (MCP)-1 production of 3D A549 cells after 4 h exposure to P. aeruginosa alone or in co-culture with R. mucilaginosa at an MOI of 10:1. NC: negative control (uninfected 3D epithelial cells in serum-free medium). Data represent mean± sem or mean, n≥3. *: p<0.05; **: p<0.001.

Article Snippet: R. mucilaginosa absolute abundance was measured by quantitative PCR (qPCR) in 82 out of 85 samples using the Qiagen Microbial DNA qPCR Assay for R. mucilaginosa (BPID00297A; Qiagen, Hilden, Germany).

Techniques: Co-Culture Assay, Negative Control

Journal: The European Respiratory Journal

Article Title: R othia mucilaginosa is an anti-inflammatory bacterium in the respiratory tract of patients with chronic lung disease

doi: 10.1183/13993003.01293-2021

Figure Lengend Snippet: Influence of Rothia mucilaginosa (Rm) on the in vivo responses to lipopolysaccharide (LPS). a) Timeline of animal infection and necropsy, b) macrophage inflammatory protein (MIP)-2 concentration (measured by ELISA), and c) number of CFU·mL −1 in mice lung homogenates after 48 h exposure to vehicle (n=7), LPS (n=18), R. mucilaginosa suspension (n=9 for R. mucilaginosa DSM20746 and n=3 for R. mucilaginosa B03V1S1C) or a combination of LPS+ R. mucilaginosa (n=18 for R. mucilaginosa DSM20746 and n=3 for R. mucilaginosa B03V1S1C). d) Lung histopathological score on haematoxylin/eosin-stained sections of the right lung. e) Lung haematoxylin/eosin-stained sections of vehicle, LPS, R. mucilaginosa B03V1S1C and LPS+ R. mucilaginosa B03V1S1C instilled mice. Scale bar: 200 μm. Vehicle: sterile alginate beads; LPS: 10 μg per 50 μL. The data presented is from two independent animal experiments for strain DSM20746 and one experiment for strain B03V1S1C. Data represent mean± sem , n≥3. *: p<0.05; **: p<0.01.

Article Snippet: R. mucilaginosa absolute abundance was measured by quantitative PCR (qPCR) in 82 out of 85 samples using the Qiagen Microbial DNA qPCR Assay for R. mucilaginosa (BPID00297A; Qiagen, Hilden, Germany).

Techniques: In Vivo, Infection, Concentration Assay, Enzyme-linked Immunosorbent Assay, Suspension, Staining, Sterility

Journal: The European Respiratory Journal

Article Title: R othia mucilaginosa is an anti-inflammatory bacterium in the respiratory tract of patients with chronic lung disease

doi: 10.1183/13993003.01293-2021

Figure Lengend Snippet: Differential expression of genes involved in inflammation by three-dimensional (3D) A549 alveolar epithelial cells exposed to Pseudomonas aeruginosa versus P. aeruginosa in combination with Rothia mucilaginosa . Quantitative PCR analysis showing average fold changes in mRNA levels of 3D A549 cells stimulated by P. aeruginosa PAO1 versus a co-culture of P. aeruginosa and R. mucilaginosa . n=3. *: statistically significant (p<0.05) fold change.

Article Snippet: R. mucilaginosa absolute abundance was measured by quantitative PCR (qPCR) in 82 out of 85 samples using the Qiagen Microbial DNA qPCR Assay for R. mucilaginosa (BPID00297A; Qiagen, Hilden, Germany).

Techniques: Quantitative Proteomics, Real-time Polymerase Chain Reaction, Co-Culture Assay

Journal: The European Respiratory Journal

Article Title: R othia mucilaginosa is an anti-inflammatory bacterium in the respiratory tract of patients with chronic lung disease

doi: 10.1183/13993003.01293-2021

Figure Lengend Snippet: Effect of Rothia mucilaginosa (Rm) on NF-κB pathway activation by Pseudomonas aeruginosa (Pa). a) Activation of the NF-κB pathway measured via luminescence of three-dimensional (3D) NF-κB reporter A549 cells. 3D cells were exposed for 4 h to P. aeruginosa PAO1 alone or in co-culture with R. mucilaginosa DSM20746. b) Semiquantitative determination (by Western blotting) of proteins ( i.e. A20, IκBα, p65 and phosphorylated (p)-IκBα) produced by 3D A549 cells stimulated with P. aeruginosa PAO1 with or without R. mucilaginosa DSM20746 for 15 min (15′), 30 min (30′), 1 h and 4 h. c) Band intensity (normalised to β-actin) of Western blot at 4 h of 3D A549 cells stimulated with P. aeruginosa PAO1 with or without R. mucilaginosa DSM20746. RLU: relative light units; NC: negative control (uninfected 3D NF-κB reporter A549 cells in serum-free GTSF-2 medium). Data represent mean± sem , n=3. *: p<0.05; **: p<0.01; ***: p<0.001.

Article Snippet: R. mucilaginosa absolute abundance was measured by quantitative PCR (qPCR) in 82 out of 85 samples using the Qiagen Microbial DNA qPCR Assay for R. mucilaginosa (BPID00297A; Qiagen, Hilden, Germany).

Techniques: Activation Assay, Co-Culture Assay, Western Blot, Produced, Negative Control

Journal: The European Respiratory Journal

Article Title: R othia mucilaginosa is an anti-inflammatory bacterium in the respiratory tract of patients with chronic lung disease

doi: 10.1183/13993003.01293-2021

Figure Lengend Snippet: The anti-inflammatory effect of Rothia mucilaginosa (Rm) is mediated by its cell-free supernatant. a) Interleukin (IL)-8 production by three-dimensional (3D) A549 epithelial cells after exposure to P. aeruginosa PAO1 (Pa) for 4 h, with or without R. mucilaginosa DSM20746 live cells, lysed cells or cell-free supernatant. b) Quantification of NF-κB pathway activation after 4 h exposure to lipopolysaccharide (LPS) (100 μg·mL −1 ) with or without R. mucilaginosa DSM20746 cell-free supernatant. The multiplicity of infection was 10:1 in all experiments. RLU: relative light units; NC: negative control (uninfected 3D epithelial cells). Data represent mean± sem , n≥3. ***: p<0.001.

Article Snippet: R. mucilaginosa absolute abundance was measured by quantitative PCR (qPCR) in 82 out of 85 samples using the Qiagen Microbial DNA qPCR Assay for R. mucilaginosa (BPID00297A; Qiagen, Hilden, Germany).

Techniques: Activation Assay, Infection, Negative Control

Journal: The European Respiratory Journal

Article Title: R othia mucilaginosa is an anti-inflammatory bacterium in the respiratory tract of patients with chronic lung disease

doi: 10.1183/13993003.01293-2021

Figure Lengend Snippet: Correlation of absolute load of Rothia mucilaginosa with pro-inflammatory parameters in induced sputum samples from bronchiectasis patients: a) interleukin (IL)-8, b) IL-1β, c) matrix metalloproteinase (MMP)-1, d) MMP-8, e) MMP-9 and f) neutrophils. Data points represent individual induced sputum samples. Correlation coefficients and p-values were calculated on ranked values when Spearman's test was used (r s ).

Article Snippet: R. mucilaginosa absolute abundance was measured by quantitative PCR (qPCR) in 82 out of 85 samples using the Qiagen Microbial DNA qPCR Assay for R. mucilaginosa (BPID00297A; Qiagen, Hilden, Germany).

Techniques: